成果發表
智慧財產
提高溶解度和免疫原性之重組蛋白質表現系統
專利名稱 |
提高溶解度和免疫原性之重組蛋白質表現系統 |
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技術領域 | 生技醫藥及相關技術 |
專利證號 | US7,524,648B2、US7,759,461B2 |
申請狀態 | 已領證 |
專利國家 | 美國、美國分割案 |
專利摘要 |
Expression system for enhancing solubility and immunogenicity of recombinant proteins. The expression system includes a protein expression vector that contains a chimeric gene encoding a chimeric protein. The chimeric protein contains three polypeptidyl fragments: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein that contains a protein transduction domain (PTD) or a fragment thereof having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment that contains a J-domain or a fragment thereof having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment that contains a target protein or polypeptide. |